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Jurnal Kimia Terapan Indonesia

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Published by Lembaga Ilmu Pengetahuan Indonesia

ISSN : 08532788 EISSN : 25277669 DOI : -

Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Materials Science & Nanotechnology

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Articles 6 Documents

Search results for , issue "Vol 2, No 1-2 (1992)" : 6 Documents clear

PERBANDINGAN Cr-51 (III) DAN Cr-S1 (VI) ANORGANIK PADA HASIL IRADIASI Cr(CO)s DAN Cr(C5H7O2)3 DENGAN NEUTRON TERMAL Sunarhadijoso Soenarjo; Said Adam; Iswandi Idris
Jurnal Kimia Terapan Indonesia Vol 2, No 1-2 (1992)
Publisher : Research Center for Chemistry - LIPI

Thermal neutron irradiation on Cr(CO)6 and Cr(C5H7O2)3 target compounds had been conducted with an average neutron flux of about 2.8 x 10(12) n.cm(-2).der(1). Both post-irradiated target compounds gave inorganic radiochromium in oxidation states of +3 and +6 which were separated by solvent extraction method: For radioactivity measurement, the trivalent species was separased from the hexavalent by hydroxide precipitation using K2CrO4 and Cr(NO)3. 9H2O carriers. The inorganic chromium content was chemically determined by spectrophotometric method without adding arry carriers. The activity of the trivalent inorganic chromium produced from Cr(CO)6 irradiation was higher than that of the hexavalent ones, but in the case of Cr(C5H7O2)3 irradiation; the activity of the hexavalent species was higher. In both cases, the specific activity of the trivalent species was higher than that of the hexavalent species. The specific activity of total inorganic chromium obtained from the irradiation of Cr(CO)6 was higher than that of Cr(C5H7O2)3.

PHOTOREACTION OF a, B - ENONES IN METHANOL IN THE PRESENCE OF TITANIUM (IV) CHLORIDE P. Lotulung; T. Sato
Jurnal Kimia Terapan Indonesia Vol 2, No 1-2 (1992)
Publisher : Research Center for Chemistry - LIPI

When cyclic a, B-unsaturated carbonyl compounds were irradiated by ultraviolet from a high pressure mercury vapour lamp at 25 °C, in alcohols in the presence of titanium chloride, a coupling reaction between the carbonyl carbon atom and the a-carbon atom of the alcohol took place, producing dihydrofurans, monomethylether, acetals or aldehides, but in the case of a-methyl substituted enone it resulted in diol monomethylether due the two times occuring methanol substitution.

THE DETERMINATION OF SUGARS BY CHROMATOGRAPHIC METHOD Sri Sumartini; Julia Kantasubrata
Jurnal Kimia Terapan Indonesia Vol 2, No 1-2 (1992)
Publisher : Research Center for Chemistry - LIPI

Experiments have been carried out to analyse sugars using TLC and HPLC methods, In the TLC method, separation of sugars was performed on silica plates impregnated with monosodium phosphate and using mixture of ethylacettuel pyridinde/water as an eluent. Whilst in the HPLC method, the use of three column types i.e. diol, RP-18 and modified silica column were tested. The results showed that TLC method was able to measure three sugars i:e. sucrose, glucose and fructose with standard deviations of 11.6%, 7,6% and 1,9%, respectively. On the other hand, the HPLC method with silica column modified by polyamine and compressed with WATERS RCM-l00, showed the best results, in which mixtures of nine sugars were well separated and measured quantitatively with good precisian.

SOLASODINE STEROID BIOCONVERSION BY MYCOBACTERIUM PHLEI DSM 43286. S. Pudjiraharti; T. A. Budiwati; J. Kantasubrata; A. T. Karossi
Jurnal Kimia Terapan Indonesia Vol 2, No 1-2 (1992)
Publisher : Research Center for Chemistry - LIPI

Bioconversion of solasodine by Mycobacterium phlei DSM 43286 was conducted to obtain intermediate compounds which might be used as precursor in the production of steroidal drugs, i:e androst-4-en-3, 17-dione (AD) and androsta-l,4-diene-3,17-dione (ADD). M. phlei was firstly grown in nutrient broth medium at 37 °C for 8.5 hours with agitation of 200 rpm. The bacterial culture thus obtained was used as starter to inoculate the conversion medium containing 0,02% solasodine as the substrate and 0.01% 8-hydroxyquinoline as inhibitor. Bioconversion was conducted for 12 days at 37 °C using the same speed of agitation. Analysis of the bioconversion products was carried oUl using samples taken periodically at a 24-hour interval by TLC and HPLC methods. TLC analysis using chloroform-ethyl acetate (80:20) as eluent, measurement of the nuvamum wavelength and molar extinction coefficient value showed that AD and ADD was not found in the fermentation product,, but other intermediau: compound might the present. However, HPLC analysis of the fermentation products using Ik'Porasil column and benzene- ethylacetatechloroform (40:80:10) as eluent, showed -peaks with retention time similar to that of AD (during the 2nd - 9th day of incubation) and, ADD (during the 5th - 6th day offermentation) and, other unknown peaks.

PERKEMBANGAN ANALISA GULA SECARA KLT DAN KCKT*) Julia Kantasubrata; Sri Sumartini
Jurnal Kimia Terapan Indonesia Vol 2, No 1-2 (1992)
Publisher : Research Center for Chemistry - LIPI

Analysis of sugars plays an important role, mainly in foods. Up to now, the search of spesific, selective, reproducible and accurate methods for sugar analysis is still being made. Sugars in foods consist of monosacharides, disacharides and trisacharides. Using conventional and spectrophotometric methods, which are based on measuremen of physical properties or chemical reactions, such individual amount of sugars can not be determined. The emergence of chromatographic techniques has initiated the development of individual sugar analysis. Thin layer chromatography (TLC) is widely used in sugar analysis, since is is simple, cheap, fast and ir has a capability to analyse several samples sinwltaneously. Commonly, lie analysis of sugars is conducted using silica gel as stationary phase. Satisfactory separation could only be produced with impregnated silica plates and multiple TLC runs. In order to reduce the separation time, new stationary phase was developed. The rapid separation of sugars has been achieved by using HPTLC plates Si 50000. In line with the development of bonded phase column, analysis of sugar using HPLC method has received considerable attention, using among others ion exchange columns, amino bonded silica phase, diol and C-18 columns. A relatively new type of sugar separation with a silica based column and eluen containing polyamine reagent has been developed by WATERS. This technique is called a SilicaAmine Modifier (SAM), in which the formation of Schiff base can be eliminated, the life time of the column becomes longer and better monosacharide separation can beproduced compared to other HPLC techniques.

EFFECTS OF MEDIUM COMPOSITION ON OXYTETRACYCLINE PRODUCTION BY STREPTOMYCES RIMOSUS ATCC 33022 Linar Z. Udin; S. Pudjiraharti; A. T. Karossi
Jurnal Kimia Terapan Indonesia Vol 2, No 1-2 (1992)
Publisher : Research Center for Chemistry - LIPI

The economical production of antibiotics to some extent depends on the availabilily of cheap substrates. The work reported in the present paper deals with the fermentative production of oxytetracycline by Streptomyces rimosus ATCC 33022 using commerciol high fructose syrup (HFS), vitamin B complex and citric acid of technical grode. The effects of concentration of high fructose syrup (0.5 - 2.5 %, v/v), commercial vitamin B complex (0.03 - 0.07 %, w/v) and the citric acid (0.34 - 1.28 %. w/v) were examined in this study. It was found that fermentation medium (medium-MHFS) containing high fructose syrup 1.0 % produced maximum activily of oxytetracycline after 4 days incubation period. Fermentation tnedium (mediwn-MBpleJ containing 0.05 % commerciol vitamin B complex showed maximum acrivily after 3 days incubation. While the addition of citric acid (0.64 %) to the fermentation medium (medium-MCA) was found optimumfor production oxytetracycline.

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